Experimental Strategies for the Identification of DNA-Binding Proteins
نویسندگان
چکیده
منابع مشابه
Experimental strategies for cloning or identifying genes encoding DNA-binding proteins.
This article describes experimental strategies for cloning or identifying genes encoding DNA-binding proteins. DNA-binding proteins are most commonly identified by electrophoretic mobility-shift assay (EMSA) or DNase I footprinting. To identify the gene encoding a protein detected by EMSA or DNase footprinting, the protein often needs to be purified and its sequence analyzed, as described here....
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ژورنال
عنوان ژورنال: Cold Spring Harbor Protocols
سال: 2011
ISSN: 1559-6095
DOI: 10.1101/pdb.top067470